MECGENE (OPC) PRIVATE LIMITED

In their own words

Product No 1. GLA from Fungus Cunninghamella elegans showed difficult biomass aggregation clumps, its jelly-like formation on the ring sparger line and Rushton Turbine impeller during the growth phase causing obstacles to the proper mass and oxygen transfer rate in process and case will be severe in the stationary phase of the growth kinetics. Such this, obstacles play crucial roles in the biomass formation ratio and as well as GLA production. In addition to this, separation of biomass from the harvested vessel, especially from impellers and sparger, are hectic to the large scale production. It is well-known fact, fungus increases the rate of the desired product when it under the aggregation states than free cells. Cunninghamella elegans is a very good “cell factory” for the mass production of microbial lipid-based product manufacturing industries. Our proposed investigation, understand the needs of support to facilitate thick jelly-like mycelia clumps of Cunninghamella elegans without causing process obstacles and favour the optimum biomass productions thereby supporting maximum GLA formation. It is possible, through the proper mass and oxygen transfer ratios to the growing cultures without forming the differential zones with the right set of the impeller and an isosceles trapezoid. Therefore, to achieve the right morphology of aggregated growth of Cunninghamella elegans for efficient GLA production, this study proposes a new type of physical structures. It has configured with following modifications. 1) A perforated SS 316 L rack of multilayered “isosceles trapezoid” like structures to achieve maximum fungal mycelia clumps. 2) Identifying the new type of impeller along with FZ, DRT, Isojet, Swingstir. 3) Shaft found moving sparger cleaner The design of perforated SS 316 L “isosceles trapezoid” structures gives physical support to the growing mycelia in the process. Implanting such these kinds of structures inside of the vessels may have profound effects in mass transfer, oxygen transfer and culture rheology. Sizing and placing of the “isosceles trapezoid” inside the vessels will be determined during process optimization. Identifying the new type of impeller suitable for fungal fermentation along with FZ, DRT, Isojet, Swingstir is the target for many chemical engineers who are working in the fermentation field. Structuring sparger cleaner without affecting the air sparging mechanism is crucial steps for the successful operation of the process. Product N0. 2 Isolation and screening of new Beta – glucosidase producing organism from various sugar and fruit waste Beta – glucosidases are the group of enzymes presents in almost all microorganisms, plants and animals. This heterogeneous glycoside hydrolyzes enzymes can break the β – glucosidic linkages of both disaccharide and oligosaccharide and glucose associated conjugates. The usage of β – glucosidase enzymes in lignocellulosic biomass based refinery industries is ever increasing one. Currently, many industries are developing more robust organism through genetic and metabolic engineering or screening the vigorous microorganisms from natural resources to meet the demand of the process requirements. Fungal based β – glucosidase are showing higher efficiency in the lignocellulosic biomass conversation. The objective of the current investigation is to isolate and screening of the effective organisms from various sugar & fruit waste from different environments. And optimisation of the media compositions and conditions which are giving the maximum yield among those selected organisms. Stress and inducer related gene expression and protein expression profile information, of about the co – upregulations and downregulations of genes during the desired product formation and its impacts in the synthesis will open the new avenue to research or another way to solve the existing problems. Methods and Techniques: The following methods and Techniques would be used in this investigation: Strain Isolation and Screening: Collected sample will be washed with double distilled sterile water and diluted samples will be inoculated on solubilized crystalline cellulose plate and carboxymethylcellulose plate. Grown culture will be transferred to MEA for pure culture isolation. Primary Screening and Secondary Screening will be done as per the established procedure. And enzyme assay will be carried out by using the p-nitrophenyl-β-D-glucopyranoside (pNPG) method. Identification of the Organism: DNA extraction, PCR amplification and DNA sequencing will be done. Media Formulation & Process Optimization: RSM and Placket-Burman methods will be used to optimize the various parameters of the process. 1 Gene Expression Profile: RNA and Protein expression will be done to identify the Gene Expression by using the Northern and Western blotting techniques. Results obtained from these blotting techniques will be confirmed through the Real Tim

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